Biotechnology and Bioinformatics (1+1)

Removal of unincorporated nucleotides by spin-column chromatography

1. Plug a 1ml syringe with glass wool, and add swollen sephadex G-50 fine resin (Pharmacia) unto top.

2. Place the syringe inside a 40ml centrifuge, spin at 1000g for 5 min.

3. Add the sample at the top of the column. Place a 1.5 ml tube before placing the column inside the centrifuge tube. Confirm that the outlet of the column hangs inside the microfuge tube.

4. Spin at 1000g for 5 min

5. Take out the microfuge tube carefully. The elute in the microfuge tube can be used without further purification.