Biotechnology and Bioinformatics (1+1)

Antigen Unmasking

For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0, then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes.

For EDTA: Bring slides to a boil in 1 mM EDTA pH 8.0 followed by 15 minutes at a sub-boiling temperature. No cooling is necessary.

a. Frozen tissue sections

Fix the tissue sections with 2-4% formaldehyde in PBS for 15 min at room temperature. Rinse the slides in PBS three times for 5 min each. Proceed for immunostaining.