Biotechnology and Bioinformatics (1+1)

2. Translating DNA to six reading frames

This tool allows the six frame translation of a nucleotide (DNA/RNA) sequences to a protein sequence in order to locate open reading frame in your sequence

Go to URL http://www.expasy.ch/tools/dna.html

Paste your sequence in the box provided and click “Translate Sequence”

You can choose from three options

- Verbose – puts Met & Stop to highlight start and stop codons

- Compact – useful if you want to use output in other programs

- Include nucleotide sequence – nucleotide sequence is above the translation

This returns a six-frame translation of your sequence. You can then choose the correct frame.