VPB 112: General Veterinary Biochemistry (1+1)

PREPARATION

Preparation of 1000 ml of Standard N/10 Oxalic acid solution

• Oxalic acid is a dibasic acid and available in crystalline form as (COOH)₂ .2 H₂0. This is taken as primary standard for acids as it can be weighed accurately.

                  Equivalent weight of Oxalic acid = (Molecular weight / 2) = (126 / 2) = 63 g

• To prepare 1000 ml of 1 N Oxalic acid solution, the amount of oxalic acid required = 63 g.
• Therefore, to prepare 1000 ml of 0.1 N Oxalic acid solution, the amount of oxalic acid required = 6.3 g.

Procedure

• Weigh 6.3 g of Oxalic acid exactly and dissolve it in 1000 ml of distilled water in a beaker and stir well.