VPB 112: General Veterinary Biochemistry (1+1)

Reactions of reducing disaccharides

EXERCISE-7: REACTIONS OF DISACCHARIDES - REDUCING

There are two types of disaccharides, viz., Reducing and Non-reducing.

Lactose and Maltose

• Perform the following tests with 1% Lactose / Maltose solution.
  • Molisch’s test
  • Fehling’s test
  • Benedict’s test
  • Barfoed’s test
  • Osazone test
• Barfoed’s test will not produce an immediate red precipitate of cuprous oxide with lactose / maltose. However, a red precipitate may be obtained on prolonged heating for about 20 minutes.

Experiment	Observation	Inference

Experiment	Observation	Inference

Experiment	Observation	Inference