ANN 111: Principles of Animal Nutrition and Feed Technology (2+1)

PRINCIPLE

• By the addition of concentrated H₂SO₄ to a feed sample and digesting it with a suitable catalyst, all the organic nutrients are split up in to their component parts. Nitrogen in the feed stuff is liberated as free ammonia and unites with H₂SO₄ to form a ammonium sulphate.
• This compound is reacted with excess of alkali and distilled. Free ammonia liberated is received in known amount of standard acid. After complete distillation, by back titrating this standard acid with standard alkali the amount of acid reacted with free NH₃ is found.
• From this nitrogen content of the feed is calculated and the protein is found out.