VMC 211: General Veterinary Microbiology (1+1)

PROCEDURE

• Place a drop of 6% glucose solution at one corner of the slide.
• Add a drop of bacterial culture to the glucose solution on the slide and mix well.
• Add a drop of India ink to the mixture and mix well again.
• Using another slide prepare a thin smear.
• Air dry the smear thoroughly and then fix for 15 seconds in methanol.
• Drain away the methanol and remove the residual methanol by passing the slide through a gentle flame.
• Stain the smear with crystal violet for two minutes
• Pour off the stain and wash the smear in running tap water.
• Air dry and examine under oil immersion.