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Species: Each species yields milk of a characteristic composition eg Cow, buffalo, dog or deer
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Breed: In general there is difference in composition among different breeds of the same species.
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Interval of milking: Longer the interval between milking more will be the quaantity of milk with lesser fat and vice versa.
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Completeness of milking: If the cow is completely milked, the test is normal. Incomplete milk leads to residual milk and thus the fat content gets reduced.
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Frequency of milking: Whether a cow is milked twice, thrice or four times a day, there is increase in volme during early lactation. ( times/day vs. 2 times/day milking. In dairy cattle, milking 3X/day results in increased milk yield, although the observed increase is variable. Generally 3X/day milking increases milk production by up to 25%. But, ~2/3 of this increase is due to better feeding and management and ~1/3 is due to decreased udder pressure. 3X/day milking must be accompanied by a compensatory feeding program; if not, then yield from the continued 3X/d milking will decline back to that from 2X/d. Three times per day milking is more beneficial in late lactation, for example during the first 4 mos. of lactation 3X/day milking increases yield 7-12% over 2X/d. In contrast, during later lactation 3X/day milking increases yield 16-90% over 2X/d. Both first lactation and older cows show increased yield in 3X/day vs. 2X/day milking. )
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Irregularity of milking: frequent changes in the time and interval of milking result in lower tests.’
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Day-to-day milking: May show variations for the individual cows.
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Disease and abnormal conditions: These end to alter the composition of milk, especially when they result in a fall in yield
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Portion of milking: fore-milk is low in fat content (less than 1 per cent), while strippings are highest (close to 10 per cent). The other milk constituents are only slightly affected on a fat free basis.
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Stage of lactation: The first secretion after calving (colostrum) is very different from milk in its composition and general properties. The change from colostrum to milk takes place within a few days.
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Yield: For a single cow; there is a tendency for increased yields to be accompanied by a lower fat percentage, and vice verse.
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Feeding: Has temporary effect only.
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Season: The percentages of both fat and solids not fat show slight but well defined variations during the course of the year
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Age: The fat percentage in milk declines slightly as the cow grows older.
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Condition of cow at calving: If the cow s in good physical condition when calving, it will yield milk of a higher fat percentage than it would if its physical condition was poor.
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Excitement: Both yield and composition of milk are liable to transient fluctuations during periods of excitement, for whatever reason.
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Administration of drugs and hormones: Certain drug may effect temporary change in the fat percentage, injection of feeding of hormones results in increase of both milk yield and fat percentage.
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