VPB 321: Animal Biotechnology (2+1)

USES OF RESTRICTION ENZYMES

• Restriction enzymes are generally used to manipulate DNA fordifferent scientific applications which includes,
• To assist insertion of genes / DNA fragments into plasmid vectors in gene cloning and subsequent protein expression experiments
• To digest genomic DNA for gene analysis by Southern blot to identify number of copies of gene present in an individual
• To digest genomic DNA for gene analysis experiments such as restriction fragment length polymorphism (RFLP) to identify the polymorphism in the population
• To distinguish gene alleles by specifically recognizing single base changes in DNA known as single nucleotide polymorphisms (SNPs).
• To digest large DNA molecules into fragments less than 300 base pairs and these fragments can then be used for sequence analysis and arranged to understand the gene structure and its regulation at molecular level.