General steps in cloning

GENERAL STEPS IN CLONING

  • Prepare the vector and the DNA to be cloned by digestion with restriction enzymes to generate complementary ends.
  • Ligate (join) the foreign DNA into the vector with the enzyme DNA ligase.
  • Introduce the DNA into bacterial cells or yeast cells by transformation.
  • Select cells containing foreign DNA by screening for selectable  markers (commonly drug resistance).

Last modified: Tuesday, 15 May 2012, 5:58 AM