VPB 321: Animal Biotechnology (2+1)

EXPRESSION SYSTEMS

• There are two main systems for the expression of recombinant protein.
• This will be either a prokaryotic (bacterial) or eukaryotic (usually yeast or mammalian cell) system.
• The choice of expression  system will decide which vector is need to clone DNA into as there are different promoters which function in E.Coli and others that work best with yeast or mammalian systems.