VPB 321: Animal Biotechnology (2+1)

POLYMERASE CHAIN REACTION 

•  PCR was invented by Kary B. Mullis in 1983.

PCR

• The PCR is an in-vitro,enzymatic amplification of a desired sequence of DNA using a pair of oligonucleotide primers.
• These primers are complementary to one end of the DNA target sequence.
• These are extended towards each other by a thermostable DNA polymerase in a reaction cycle of three steps; denaturation, primer annealing and polymerization.