Assembling of sequenced part of a gene

ASSEMBLING OF SEQUENCED PART OF A GENE
  • For publication purposes, each sequence of a gene has to be confirmed in both directions.
  • To accomplish this, the gene has to be sequenced with forward and reverse primers.
  • Since it is only possible to sequence a part of 750 till 800 bases in one run, a gene of, for example 1800 bases, has to be sequenced with internal primers.
  • When all these fragments are sequenced, a computer program such as DNA star tries to fit the different parts together and assembles the total gene sequence.
Last modified: Friday, 24 September 2010, 10:54 AM