VPP 321: Avian Pathology (1+1)

DILUTING FLUID

• For erythrocyte counts, dilutingfluid described already can be used.

Make the following stock solution

• A. Sodium citrate 2%
• B. 0.1% Gentain violet in Ringer’s solution
• C. 0.l% Brilliant cresyl blue in Ringer’s solution
• D. Neutral formalin
• The above 4 solutions must be made and kept separately as stock solutions. From these the working solution is made daily before making the counts as follows:
  • A . 1ml
  • B. 2m1
  • C. 3ml
  • D. 3 drops.
• Mix and filter

Ringer’s solution has the following composition

• Sodium chloride -0.7 gms
• Sodium bicarbonate- 0.03 gm
• Potassium chloride- 0.026 gm
• Calcium chloride- 0.003 gm
• Distilled water- 100 ml
• With the above solution, erythrocytes take a very light blue color and clearly seen. 
• Granulocytes are prominent.
• Thrombocytes are also clearly visible and uniformly distributed and can be easily counted. 
• Moreover, with the above fluid the heterophils, monocytes and lymphocytes can be identified easily.