VLD 421: Veterinary Clinical Biochemistry and Laboratory Diagnosis-II (0+1)

MATERIALS FOR VIRAL DISEASES

S.No	Disease	Clinical Materials	Preservatives
1	FMD	Vesicular fluid. Epithelial tissue collected from unruptured or recently ruptured vesicular lesion in foot, tongue, mouth.gum,iterdigital space,teat and udder . Blood, esophageal or pharyngeal fliud and throat swab . Gum scrappings . Paired serum sample .	50% glycerol saline or Phosphate buffered glycerol  saline.
2	Bovine viral    diarrhoea and   Mucosal disease	Defibrinated blood , paired serum samples. Dead animals: spleen, and mesenteric lymph nodes .	50% glycerol saline or Phosphate buffered glycerol saline.
3	IBRT (Infectious   bovine rhino    tracheitis)	Live animal: Nasal swab or swabs from external genitalia in case of vulvo vaginitis or Balanoposthitis. semen, prepucial washing. Dead animals : Mucus membrane of Respiratory tract, portion of tonsil, Lung, Bronchial LN, Fetal Liver, Lung, spleen, Kidney, cotyledons .	On ice or Cell culture medium containing antibiotics & fetal bovine serum
4	Blue tongue	Live animal: Blood in an anticoagulent at peak of temperature, paired serum sample. In dead animal :Spleen, liver,bone marrow and mesenteric lymph node on ice.	50% glycerol saline
5	Peste des petits   ruminants-PPR	Live animals: Swabs from conjunctival discharge, nasal and buccal mucosa, whole blood in anticoagulant solution and paired serum sample . In dead animals: Lymph node, lung, spleen and intestinal mucosa.	Minimum essential  medium (Commonly used for tissue culture) Medium 199 /RPMI 1640
6	Sheep &Goat Pox	Skin papules, scab from affected portion, lung, lymph nodes and whole blood in EDTA or buffy coat .	Blood in anticoagulant, other materials – 50 %glycerol saline.
7	Swine fever	Heart blood,piece of Lymph node, Spleen,heart,distal ileum and kidney.	On ice or Equal amount of glycerol & 0.4M phosphate buffer with antibiotics
8	Equine infectious    anemia	Blood.	-
9	Canine Distemper	Blood at high temperature ,swab from conjuntiva,nasal discharge,scraping from gums,Pustules or fluid from the affected animals Dead animals:Tachea,lung,brain,kidney,mediastinal lymphnode,liver and urinary bladder scraping.	In Hank's balanced salt solution or in ice.
10	Rabies	Animal head / Brain or impression smear (Thalamus, pons, medulla, Hippocampus or cerebellum)	50% glycerol saline or portion of brain preserved over ice.
11	New Castle   Disease	Live birds: Tracheal, conjunctival, throat and cloacal swab, dropping on ice. Serum Dead birds: Oral nasal swab, Lung, Kidneys, intestinal contents, proventriculus, spleen, liver, heart& brain. Serum from affected or convalescent birds.	Isotonic PBS with antibiotics or 50% glycerol saline.
12	Infectious Bursal   Disease	Cloacal bursa, Kidney, spleen in PBS on ice, serum.	50% glycerol saline or in ice
13	Marek’s disease	Live birds: Heparinised Blood, serum from convalescent bird , feather follicle from affected bird in 0.2% EDTA. Dead birds: Lymph nodules from visceral organs, liver, Gonads, spleen, kidney, lung & proventriculus.	50% glycerol saline
14	Avian Influenza	Swab from Cloaca, oronasal area,Faeces and sinus exudate. Dead birds: peritonelal fluid , intestinal contents, , trachea, lungs, bursa, spleen, kidney, brain, liver & heart.	Isotonic PBS with antibiotics
15	Avian infectious   bronchitis	Live birds: Swab from cloaca, trachea Dead birds:trachea, bronchi oviduct, spleen, airsac, kidney & caecal tonsil.	50% glycerol saline or preserved over ice
16	Egg drop   syndrome-76	Live bird: serum Deadbird :spleen,kidney,trachea,lung,liver,brain,caecal tissue and bursa.	Preserved on dry ice or 50% glycerol saline
17	Avian leucosis   complex	Blood,serum, cloacal swab, muconium from from day old chick,egg albumin,and embryos . Dead Birds: liver, spleen, kidney, gonads and bone marrow.	Tissue in 10% formalin on ice.
18	Fowl pox	Dry scab or swab from lesions on mouth esophagus, larynx & trachea .	10% PBS suspension
19	Canine parvo   viral enteririts	Heparinised blood, faeces. Dead animal: Faeces, liver, spleen, kidney, lymphnode.	on ice or organs in PBS on ice.
20	Chicken anemia   virus	Liver, thymus, bone marrow and rectal contents .	Ice packed
21	Hydropericardium syndrome	Liver,spleen, pericardial fluid, heart and kidney.	PBS on ice
22	Duck plague	Liver, spleen, kidney, heart, esophagus, cloacal bursa, intestine annular bands.	On dry ice
23	Duck hepatitis   virus	Liver.	PBS on ice