Preparation of sample

Preparation of sample

  • Immediately after excision from the animal, drop approx. 1 g of tissue into a previously weighed test tube containing 3 ml of 30% KOH.
  • After delivery of the sample, reweigh the tube and contents, and note down the weight of the sample.
  • Digest the tissue by heating the tube for 20 min. in a boiling water bath, cool, transfer quantitatively to a 50 ml volumetric flask.
  • Mix the contents of the flask thoroughly and make up to 50ml in a volumetric flask.
  • Pipette out 10ml of this and dilute to 100ml in a volumetric flask. Use this as sample solution.
Last modified: Monday, 14 November 2011, 5:56 AM