Reagents
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Lipase substrate: Olive oil, 50% (v/v) and sodium azide, 0.1% added as preservative. Shake vigorously for a minimum of 20 sec. before using. Store at room temperature (18- 26ºC). Do not freeze.
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Trizma buffer (Tris (hydroxymethyl) amino methane) 0.2 mol. pH 8.0 at 25ºC: Add 0.1% sodium azide as preservative. Store at room temperature (18-26ºC). Discard if turbidity develops.
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Thymolphthalein indicator: Thymolphthalein 0.9% (W/V) in ethanol. Store tightly capped at room temperature (18-26ºC).
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Ethanol : 95%
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Sodium hydroxide 0.05 N: Prepare 0.1NaOH solution and standardize by titration against a standard 0.1 acid. Using the formula V1 N1 =V2 N2 find out the normality.
Then prepare exactly 0.05 N NaOH solution. Store tightly capped in pyrex or polyethylene bottle.
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Last modified: Monday, 14 November 2011, 5:25 AM