Reagents

Reagents

1. Gel filtration buffer : 0.1M Phosphate buffer solution pH 7.0.

2. Sample mixture : Dextran – Mol wt 2,000,000 Da -10mg/ml

Hemoglobin –Mol wt   64500 Da -10mg/ml

Vitamin B12 – Mol wt  376 Da. 10mg/ml

Mix1:1:1 mix of all the above

Last modified: Monday, 19 March 2012, 6:36 AM