Procedure
- Fix the column vertically to a stand.
- Equilibrate the column with 4ml Gel filtration buffer. Drain out the buffer completely.
- Load 0.2ml of the sample onto the column, along the sides of the column.
- Allow the sample to sink completely.
- Allow the buffer to flow out completely.
- Keep filling the column with buffer, till all the coloured biomolecules have eluted out (approx. 20 ml buffer is needed).
- Collect the coloured fractions in different tubes.
- Fix the bottom cap and then the top cap to stop the flow of buffer.
Observation
- Note down the time and order in which various molecules leave the column.
(Store the column at 4°C for next use.)
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Last modified: Monday, 19 March 2012, 7:31 AM