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Determination of specific activity of lysozyme
Specific activity of lysozyme in crude and eluted samples is calculated by comparing the OD readings of the samples with that of standard lysozyme, whose specific activity is known.
Activity in U/mg = ∆A450 / min. of the test x activity of the standard* ---------------------------------------------------- ∆A450 / min. of the standard
Where ∆A450 is the difference in A450 between 0 sec and 60 sec * Activity of standard is 48,000 U/mg |
Last modified: Saturday, 16 June 2012, 10:43 AM