Principles of Biochemistry

Measurement of yield

Yield = Protein concentration of eluate x total volume of eluate = ----------mg

Result

 Calculate and report fold purification of the enzyme lysozyme

	C1	C2	C3	C4	C5
	Total volume	Protein conc. (mg/ml)	Total protein (in mg) C1xC2	Specific Activity (U/mg)	Total Yield (U) C3xC4
Crude sample
Eluate