Elementary plant Biochemistry and Biotechnology (2+1)

Energetics of glycolysis

• From glucose, two molecules of glyceraldehyde 3-phosphate are formed in the second stage of glycolysis from which two molecules of pyruvate are obtained as end products of glycolysis.
  
• Hence energetic of glycolysis is calculated by taking into account two molecules of glyceraldehyde 3-phosphate.
Energetics of glycolysis Stages/steps	Enzyme	Method of high energy bond formation	No. of Formed
ATP
Formation of 1,3-bisphospho glycerate from glyceraldehydes 3-phosphate	Glyceraldehyde 3-phosphate dehydrogenase	Respiratory chain oxidation of 2 NADH	5
Stage 2 Formation of 3 phosphoglycerate from 1,3 bisphospho glycerate	Phosphoglycerate kinase	Phosphorylation at subtrate level	2
Stage 3 Formation of pyruvate from phosphoenol pyruvate	Pyruvate kinase	Phosphorylation at subrate level	2
Allowance for consumption of ATP by reactions catalysed	Hexokinase and phosphor fructose kinase		2
Number of ATP molecules enerated by catabolism of one molecule of glucose under aerobic conditions			7
Number of ATP molecules generated by the catabolism of one molecule of glucose under anaerobic conditions			2