Elementary plant Biochemistry and Biotechnology (2+1)

Stage 3. Rooting of shoots

• Somatic embryos carry a pre-formed radicle and may develop directly into plantlet. However, these embryos often show very poor conversion into plantlets.
  
• They require an additional step of maturation to acquire the capability for normal germination.
• Adventitious and axillary shoots developed in cultures in the presence of a cytokinin generally lack roots.
  
• To obtain full plants the shoots must be transferred to a rooting medium which is different from the shoot multiplication medium, particularly in its hormonal and salt compositions.
  
• For rooting, individual shoots measuring 2 cm in length are excised and transferred to the rooting medium