Elementary plant Biochemistry and Biotechnology (2+1)

Plant Transformation

• Plant transformation has become widely adopted as a method to both understand how plants work and to improve crop plant characteristics. Plant transformation depends on the stable introduction of transgene(s) into the plant genome. Various methods have been developed to achieve this and many plant species have been successfully transformed. As an initial step the gene of interest is isolated and then integrated into a suitable vector. The recombinant vector is used for transformation in variety of methods. These methods were broadly grouped into two classes. 1. Direct Gene transfer 2. Indirect Gene transfer.