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Stages of Micropropagation
Micro propagation is an integrated process in which cells, tissues or organs of the selected plants are isolated, surface sterilized and incubated in growth promoting, sterile medium and environment to produce a large number of plants. The different stages are: Stage 0: Selection of mother plant for explants isolation: The mother plant from which explants has to be excised should be a.) A certified and true to type representative of the desired species and or cultivar. Stage1: Explant establishment in culture medium: During this stage the explant is cultured in a suitable culture medium, preferably agar based media for tissue activation and multiplication.b.) Healthy and free from insect pest and disease c.) Should be quite vigorous. Stage2: Proliferation and multiplication: In this stage, repeated sub-culturing is done to encourage more proliferation, which largely depends upon the combination of growth regulators (Plate 13.1). The duration of this stage is unlimited and largely depends on the choice of propagator. Stage 3: Plant establishment and rooting: In this stage the selected plants are forced for root formation, which can be achieved by media modification and modifying the concentration of growth regulators. The concentration of cytokinins and sugars are reduced and concentration of auxins and light intensity in the laboratory is increased to start with photosynthesis and other physiological activities. Plate 13.1 Proliferation and multiplication of plants Plate 13.2. Hardening of tissue culture plants Plate 13.3 Hardening of plants |
Last modified: Thursday, 20 September 2012, 8:08 AM