Principle

PRINCIPLE

Total Proteins

  • Proteins in serum react with copper of Biuret Reagent in alkaline medium to form a blue purple colored complex with absorption maximum at 550 nm.

Albumin

  • Albumin in serum binds with the dye Bromocresol Green (BCG) at pH 3.8 to form a green colored complex, the absorbance of which is measured at 600 nm.
Last modified: Saturday, 26 November 2011, 6:16 AM