Diagnosis
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In case of food poisoning identification of the enterotoxin is of diagnostic use.
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CPS produces typical reaction in the Hotis test of milk samples.
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These organisms produced green or greenish brown colonies with white centres after the milk samples are incubated for 16-20 hours.
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DNA test and protein A tests are also employed.
Pathogenicity test
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Coagulase test: When culture added to Rabbit plasma, fibrinogen is converted to fibrin by coagulase enzymes.
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In this test, a suspension of staphylococci is mixed with rabbit plasma either on a slide or in a small tube.
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The slide test detects the presence of a bound coagulase or clumping factor on the bacterial surface.
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A positive reaction is indicated by clumping of bacteria within 1 to2 min.
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The tube test detects the free coagulase or staphylocoagulase, which is secreted by bacteria into the plasma.
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It is the definitive test for coagulase production and positive reaction is indicated by clot formation in the tube following incubation at 37ÂșC for 24 hrs. (Click here for visual)
Phage typing
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Last modified: Monday, 4 June 2012, 4:16 AM