Veterinary Preventive Medicine-II

DIAGNOSIS

• Based on history, clinical findings, seasonal occurrence
• Samples to be collected -unclotted blood from febrile animals or fresh spleen and lymph node collected at post mortem
• Virus isolation in unweaned mice or growing in susceptible cell cultures or in embryonated eggs (i/v or yolk sac routes).
• The virus propagated in primary cultures of calf kidney, adrenal and testis, HELA cell cultures and also BHK21. CPE within 24-72 hours, which consists of foci of enlarged and retractile cells
• Other tests – CFT, SNT, Plaque inhibition test, Microgel diffusion test, FAT, AGID, ELISA

Differential diagnosis

• Foot and mouth disease
• Malignant catarrhal fever
• Photosensitization