Collection and Preservation of Biopsy Samples
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Bone marrow
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Since bone marrow cells degenerate after death it should be collected from live animals. Bone containing active red marrow is selected and collected using biopsy needle.
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In horse and cow the external angle of ilium, sternabra in sheep and sternum or ilium in dog is suitable for puncture.
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The bone marrow smear is fixed in methyl alcohol for 2 minutes.
Liver biopsy
Site
Cattle /font>
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11th intercostal space
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Horse
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11th or 14th intercostal space
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Dog
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8th to 10th intercostal space or from a ventral midline incision
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The technique of liver biopsy can be dangerous, if performed by unskilled hands it may lead to severe haemorrhages.
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It is also essential that asepsis to be maintained otherwise there is a risk of peritonitis.
Renal biopsy
- Renal biopsy in dogs and cats may be carried out by Franklin modified Vim –Silvermann needle or by keyhole surgery.
Consequences
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Haemorrhages might be a problem following renal biopsy.
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Hence, it is advisable to evaluate the haemostatic status of the patient prior to surgery.
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Last modified: Saturday, 24 September 2011, 7:33 AM