Systematic Veterinary Virology

CULTIVATION

• APMV-1 can be readily cultivated in embryonated eggs. The preferred route is allantoic cavity route. 9-11 days old embryos are preferred for cultivation.
• Virulent strains cause death of embryos in 24 hours with haemorrhages through out the body particularly in occipital region. Lentogenic strains do not cause death.
• Presence of virus is usually confirmed by HA and HI test using specific serum. The virus also grows in primary cells like Chicken embryo fibroblast and pig kidney cells. The virus also grows in BHK21 cell line.