SOIL AND PLANT ANALYSIS (1+1)

Principle

The sample is vaporized by aspiration of solution into a flame or evaporation from electrically heated surface (temperature range: 1800 – 31000 K). At this condition where the individual atoms co-exist, a beam of light is passed through them. The atoms will absorb in the visible and ultraviolet region resulting in changes in electronic structure (excited state). So, the resultant light beam coming out of the sample will be missing the light in the corresponding wavelength, which is a measure of the characteristics of the sample.

Atomic Absorption Spectrophotometry is designed to determine the amount (concentration) of an object element in a sample, utilizing the phenomenon that the atoms in the ground state absorb the light of characteristic wavelength passing through an atomic vapor layer of the element.