Laboratory Diagnosis

LABORATORY DIAGNOSIS

Isolation and identification

  • Clinical materials: Nasopharyngeal swabs and nasal or tracheal washings are the ideal clinical materials for isolation of viruses.
  • Isolation systems: Embryonated eggs (allantoic or amniotic routes) and MDCK cell line.
  • Identification of antigen:
    • Demonstration of HA activity using chicken or guinea pig RBCs.
    • Neuraminidase typing
    • Antigen capture ELISA using monoclonal antibody against nucleoprotein
    • RT-PCR
Last modified: Wednesday, 29 September 2010, 5:02 AM