Systematic Veterinary Virology

CULTIVATION

• EIV are cultivated in embryonated eggs and cell culture systems. 10-11 days old embryonated eggs are used for cultivation of EIV. The routes are allantoic or amniotic routes and the infected eggs are incubated for 2-3 days.
• The eggs are chilled after 2-3 days and the presence of EIV is confirmed by demonstration HA property using chicken or guinea pig RBCs. The cell culture system commonly used is MDCK (Madin–Darby canine kidney cell line).
• The cells are incubated for 7 days. The presence of EIV is confirmed by demonstration of HA property in tissue culture fluid using chicken or guinea pig RBCs and haemadsorption.