Systematic Veterinary Virology

CULTIVATION

• The preferred cell culture systems for virus growth are Madin–Darby Canine Kidney (MDCK), swine kidney, swine testicle or swine lung epithelial cell lines. Specific cytopathic effects appear 7 days of incubation. The virus also grows well in embryonated eggs (9-11 days old).
• The eggs are incubated for four days. The presence of virus is confirmed by HA test using chicken or turkey RBCs.