Systematic Veterinary Virology

Laboratory diagnosis - Isolation and identification

ISOLATION AND IDENTIFICATION

• Is attempted FAT gives an uncertain result or when the FAT is negative in the case of known human exposure.
• Mouse inoculation test: The infected mice develop paralysis and death occurs within 28 days. Upon death, the brain should be examined for the presence of the virus by immunofluorescence.
• Cell culture system: Neuroblastoma cell lines, e.g. CCL-131 is commonly used for isolation of rabies virus. Presence of rabies virus in the cells is revealed by the FAT.