Systematic Veterinary Virology

CULTIVATION

• BEFV are cultivated in embryonated eggs, suckling mice, vero cells and cattle. BEFV are cultivated through intravenous inoculation into chicken embryos.
• Lesions are not characteristic and infected embryos die. BEFV are identified by immunofluoresence in heart, brain, lung and liver of chicken embryos at 1-5 days and in lung and liver of one-day-old chickens at 1-2 days.