Systematic Veterinary Virology

LABORATORY DIAGNOSIS

• Isolation and identification
• Clinical materials: Liver is ideal clinical material for isolation of virus.
• Systems for isolation: Ducklings, Duck or chicken embryonated eggs and primary cell culture like Duck embryo liver.
• Changes / CPE / Lesions: As mentioned under cultivation.
• Immunological tests
• The virus can be identified by neutralization with specific antisera or by inoculation into both susceptible and immune ducklings.
• Types II and III viruses are not neutralized by classic Type I antiserum.