Systematic Veterinary Virology

CULTIVATION

• The virus is cultivated in a number of bovine monolayer cell cultures (e.g. kidney, lung, testis or turbinate). Both biotypes grow well in cell cultures.
• Noncytopathogenic BVDV is a common contaminant of fresh bovine tissue. Hence, the cell cultures from bovine origin used for BVDV cultivation should be checked for residual contamination.
• Now, such problems are overcome by the use of continuous cell lines, which can be obtained as BVD-free cell lines.
• The foetal bovine serum used in cell culture should also BVDV and antibodies against BVDV. The presence of virus in the cell culture is confirmed by immunoperoxidase technique, ELISA and nucleic acid identification methods.