Systematic Veterinary Virology

LABORATORY DIAGNOSIS

• Isolation and identification (Accepted for International trade)
• Clinical materials: Whole blood, swabs from mucosal or nasal surfaces, semen and lymphnodes.
• Isolation systems: Bovine monolayer cell cultures from kidney, lung, testis or turbinate.
• Antigen identification
• Microplate immunoperoxidase method
• Double antibody sandwich ELISA
• RT-PCR
• Direct identification of BVDV from tissue sections
• Clinical materials: Tissues from lymph nodes, thyroid gland, skin, brain, abomasum and placenta are ideal for direct identification of BVDV.
• Techniques
• Immunoperoxidase method using monoclonal antibodies
• RT-PCR
• In situ hybridisation with enzyme-linked riboprobes