Cultivation

CULTIVATION
  • The virus grows well in embryonated chicken eggs and cell culture systems.
    • In embryonted chickens the preferred route of inoculation is yolk sac. The eggs should be incubated in a humid chamber at 33.5C. The embryos die between 2 and 7 days. Infected haemorrhages have cherry red appearance due to extensive haemorrhages. The presence of virus can also be confirmed by immunofluorescense, immunoperoxidase or antigen capture ELISA tests. 
    • BTV also grow in cell culture systems like baby hamster kidney (BHK)-21, African green monkey kidney (Vero) and Aedes albopictus (AA) cells. The cytopathic effects should be confirmed by antigen-capture ELISA, immunofluorescence, immunoperoxidase, or virus neutralisation (VN) tests.
Last modified: Thursday, 30 September 2010, 7:06 AM