Systematic Veterinary Virology

CULTIVATION

• Cell culture: AHSV can be cultivated in baby hamster kidney-21 (BHK-21), monkey stable (MS) and African green monkey kidney (Vero).
• Embryonated chicken eggs (ECE): The virus grows in chicken embryos but no lesions or death of embryo take place. The virus can also be cultivated in chicken embryo fibroblast, but no CPE are produced.
• Lab animals: The virus can be cultivated in newborn mice and guinea pigs. In newborn mice the viruses are inoculated through intracerebral route.