Systematic Veterinary Virology

SEROLOGY

• The following serological tests are used to identify antibodies against AHSV. They are
• Indirect ELISA (Accepted for international trade) - VP7 protein isn used as antigen for AHSV antibody determination. This test has high degree of sensitivity and specificity. 
• Complement fixation test (Accepted for international trade)
• Immunoblot test: The binding of antibodies to viral proteins separated by electrophoresis and transferred to nitrocellulose paper is used for the determination of anti-AHSV antibodies.
• NS3 ELISA: This test distinguish between infected horses and horses vaccinated with the inactivated purified AHSV serotype 4 vaccine.
• Haemagglutination inhibition test
• Virus neutralization test: Done to serotype AHSV