Systematic Veterinary Virology

SEROLOGY

• AGID: The AGID test is the most useful of the serological tests for the detection of specific antibodies in serum
• Quantitative AGID test: The AGID test is used to measure antibody levels by using dilutions of serum in the test wells and taking the titre as the highest dilution to produce a precipitin line. This can be very useful for measuring maternal or vaccinal antibodies and for deciding on the best time for vaccination.
• Virus neutralization test: VN tests are carried out in cell culture. The test is more laborious and expensive than the AGID test, but is more sensitive in detecting antibody. This test is also useful for evaluating vaccine responses or for differentiating between IBDV 1 and 2 serotypes
• ELISA: This is the preferred test for identification and quantification of antibodies against IBD.