Systematic Veterinary Virology

CULTIVATION

• Cell culture: Capripoxvirus will grow in tissue culture of bovine, ovine or caprine origin. Primary or secondary cultures of lamb testis (LT) or lamb kidney (LK) cells are considered to be the most susceptible. The CPE include retraction of the cell membrane from surrounding cells, and eventually rounding of cells and margination of the nuclear chromatin. The other characteristic CPE is the eosinophilic intracytoplasmic inclusion bodies, which are variable in size but up to half the size of the nucleus and surrounded by a clear halo. Syncytia formation is not a feature of capripoxvirus infection.
• Animal inoculation: Lambs are used for cultivation of SGPV.