Systematic Veterinary Virology

CULTIVATION

• The virus is cultivated in Chicken kidney or or duck embryo fibroblasts. The cytopathic effects, termed plaques, appear within 3–5 days and can be enumerated at about 7–10 days. They also produce intranuclear inclusion bodies. In ECE, the virus produce pock lesions when infected through CAM route. SPF chickens are also used for cultivation of the virus.