Systematic Veterinary Virology

DIAGNOSIS

• Field diagnosis
• Based on clinical symptoms in correlation with the production performance.
• Isolation and identification
• Clinical materials: Abnormal eggs and pouch shell glands
• Isolation system: Embryonated duck eggs or duck- or chick-embryo liver cell cultures or susceptible antibody free chickens
• Serological tests
• HI test
• ELISA
• Serum neutralization test
• Double immuno diffusion test
• Nucleic acid identification methods
• PCR
• Differential diagnosis
• Newcastle disease
• Avian Influenza
• Infectious bronchitis