Leishman's staining

LEISHMAN'S STAINING TECHNIQUE
  • Prepare and dry the thin blood smear in air. 
  • Cover the smear with a known quantity of the stain (normally, 8-10 drops) and allow to remain for 1 minute.
  • Add double the quantity of neutral distilled water and mix it well with the stain by means of blowing air through a pipette and allow to remain for 10-15 minutes.
  • Wash off the stain quickly and allow the slide to dry in a vertical position and then examine under oil immersion objective.

Last modified: Sunday, 11 September 2011, 4:43 AM