Giemsa staining

GIEMSA STAINING TECHNIQUE

Procedure

  • Fix the blood film in absolute methyl alcohol for 2 minutes. This can be done by dipping the smear briefly in a coplin jar containing methyl alcohol.
  • Let the smear to dry.
  • Stain in a 1 in 10 dilution of Giemsa’s stain for 20 minutes. (Add 1 ml of stock Giemsa’s to 9 ml of neutral buffered water)
  • Wash the smear, air dry it and examine under oil immersion objective for detecting the parasite.
Last modified: Sunday, 11 September 2011, 4:44 AM