Buffer
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A variety of organic and inorganic buffers have been used for deep freezing of semen.
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But an ideal buffer should prolong the life if spermatozoa at room temperature penetrate cells and acts intracellular buffer, less toxic in the critical temperature during freezing and better clarity under microscope.
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Tris is commonly called as universal diluents which prolongs the life of sperm at room temperature, penetrate sperm and acts as intra cellular buffer, less toxic at the critical low freezing temperature.
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Preparation of buffer
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Last modified: Monday, 4 June 2012, 10:14 AM