Veterinary Andrology and Reproductive Techniques

DISTILLED WATER

Use autoclaved triple distilled water or Milli-Q water for buffer and diluent preparation.

• Triple distilled or Milli-Q purified water should be used for preparation of dilutor.

• The distilled water should be sterilized by autoclaving at 15 psi for 15 minutes.
• For storing the distilled water autoclaved sterile glass wares should be used.
• The triple distilled or Milli-Q water stored for more than one week should not be used.